3 single cell cdna 461 libraries (10X Genomics)
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10X Genomics
3 single cell cdna 461 libraries
3 Single Cell Cdna 461 Libraries, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries/pm42271351-235-13-24
Average 86 stars, based on 1 article reviews
3 Single Cell Cdna 461 Libraries, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+libraries/pm42271351-235-13-24
Average 86 stars, based on 1 article reviews
3 single cell cdna 461 libraries - by Bioz Stars,
2026-09
86/100 stars
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Sequencing:Article Title: Mapping glioblastoma's isoform diversity using long-read single-cell analysis. Article Snippet: .. Among these, 19 were male (70.4%; median age: 63 years) and 8 were female (29.6%; median age: 57 years). Article Title: How Single-Cell Transcriptomics of Hydractinia Is Informing the Evolution of Cnidarian Sensory Systems. Article Snippet: Fixed single cell samples were diluted to 1,000 cells/μL and shipped on dry ice to the National Institute of Health Intramural Sequencing Center (Bethesda, MD), where cells were thawed, spun, resuspended and loaded onto the 10X Genomics platform for encapsulation, with the capture target of 6,000- 9,000 cells per sample. .. Sequencing libraries were prepared according to the standard 10X Genomics V3 chemistry protocol. Article Title: A Multi-Step Immune-Competent Genetically Engineered Mouse Model Reveals Phenotypic Plasticity in Uveal Melanoma Article Snippet: Single cells, reagents, and 10X Genomics gel beads were encapsulated into individual nanoliter-sized Gelbeads in Emulsion (GEMs), and reverse transcription of polyadenylated mRNA was performed in each droplet at 53°C. .. The Single Cell:Article Title: Modeling the role of urokinase-type plasminogen activator, uPA, and circulating cancer-associated fibroblasts (cCAFs) in breast cancer cell extravasation. Article Snippet: Several samples were made and compared for our analysis: 1) MDA-MB231, CAF23, and DT28 single cells grown in 2D with their standard media, 2) CAF23 mono-clusters, MDAMB231 mono-clusters, and DT28 mono-clusters grown in 3D with Mammocult media for 48 h, and 3) CAF23/MDAMBA231 co-clusters and CAF23/DT28 co-clusters grown in 3D with Mammocult media for 48 h. Since not all cells in the clustering assay became part of a cluster, we used Pluriselect filters to remove non-clustered cells, and then used Accutase to disrupt the clusters. .. A single-cell suspension of each sample was labeled with Hashtag Oligos (HTOs) (Supplemental Table 1) in order to pool samples, and Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease. Article Snippet: Single-cell T-cell receptor repertoire sequencing Total CD4+ T cells from the cervical lymph nodes (cLN) of Ctrl and pSjD model mice were purified with CD4 (L3T4) MicroBeads (Miltenyii BioThech, Cat# 130-117-043). .. The Article Title: A Multi-Step Immune-Competent Genetically Engineered Mouse Model Reveals Phenotypic Plasticity in Uveal Melanoma Article Snippet: Single cells, reagents, and 10X Genomics gel beads were encapsulated into individual nanoliter-sized Gelbeads in Emulsion (GEMs), and reverse transcription of polyadenylated mRNA was performed in each droplet at 53°C. .. The Suspension:Article Title: Modeling the role of urokinase-type plasminogen activator, uPA, and circulating cancer-associated fibroblasts (cCAFs) in breast cancer cell extravasation. Article Snippet: Several samples were made and compared for our analysis: 1) MDA-MB231, CAF23, and DT28 single cells grown in 2D with their standard media, 2) CAF23 mono-clusters, MDAMB231 mono-clusters, and DT28 mono-clusters grown in 3D with Mammocult media for 48 h, and 3) CAF23/MDAMBA231 co-clusters and CAF23/DT28 co-clusters grown in 3D with Mammocult media for 48 h. Since not all cells in the clustering assay became part of a cluster, we used Pluriselect filters to remove non-clustered cells, and then used Accutase to disrupt the clusters. .. A single-cell suspension of each sample was labeled with Hashtag Oligos (HTOs) (Supplemental Table 1) in order to pool samples, and Labeling:Article Title: Modeling the role of urokinase-type plasminogen activator, uPA, and circulating cancer-associated fibroblasts (cCAFs) in breast cancer cell extravasation. Article Snippet: Several samples were made and compared for our analysis: 1) MDA-MB231, CAF23, and DT28 single cells grown in 2D with their standard media, 2) CAF23 mono-clusters, MDAMB231 mono-clusters, and DT28 mono-clusters grown in 3D with Mammocult media for 48 h, and 3) CAF23/MDAMBA231 co-clusters and CAF23/DT28 co-clusters grown in 3D with Mammocult media for 48 h. Since not all cells in the clustering assay became part of a cluster, we used Pluriselect filters to remove non-clustered cells, and then used Accutase to disrupt the clusters. .. A single-cell suspension of each sample was labeled with Hashtag Oligos (HTOs) (Supplemental Table 1) in order to pool samples, and Generated:Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease. Article Snippet: Single-cell T-cell receptor repertoire sequencing Total CD4+ T cells from the cervical lymph nodes (cLN) of Ctrl and pSjD model mice were purified with CD4 (L3T4) MicroBeads (Miltenyii BioThech, Cat# 130-117-043). .. The Article Title: A Multi-Step Immune-Competent Genetically Engineered Mouse Model Reveals Phenotypic Plasticity in Uveal Melanoma Article Snippet: Single cells, reagents, and 10X Genomics gel beads were encapsulated into individual nanoliter-sized Gelbeads in Emulsion (GEMs), and reverse transcription of polyadenylated mRNA was performed in each droplet at 53°C. .. The Mouse Assay:Article Title: ACNP 64 th Annual Meeting: Panels, Mini-Panels and Study Groups. Article Snippet: .. Methods: snRNA-Seq: Mice (60) self-administered OXY (12 days) prior to introducing air-puff paired to drug intake; following classification as punishment sensitive (PS) or resistant (PR), the VP was collected and other:Article Title: Resolving spatial subclonal genomic heterogeneity of loss of heterozygosity and extrachromosomal DNA in gliomas. Article Snippet: For cohort A, the Qiagen blood protocol (Qiagen, #69504) was used with minor adjustments to account for 100mL initial input volume. Article Title: Targeting iron-associated protein Ftl1 in the brain of old mice improves age-related cognitive impairment. Article Snippet: Libraries were sequenced on an Illumina NovaSeq 6000 S2. |